RESOURCES

RESOURCES

PROTEIN AND PROTEOMICS CENTRE (PPC)
A centre for research on proteins, peptides and functional genomics

The Protein and Proteomics Centre (PPC) is a multi-user core facility focused on advanced research in proteins with an emphasis on mass spectrometry. Areas of expertise include biomarker discovery, proteomics, quantitative proteomics, and structural mass spectrometry (Amide Hydrogen/Deuterium Exchange MS and Ion Mobility MS). PPC works closely with academic and industrial partners in biomedical research.

Services

At the Protein and Proteomics Centre, our goal is to provide state-of-the-art mass spectrometry services and expertise while expanding MS applications across the Life Sciences spanning biology, chemistry and pharmacy.

We aim to build on our status as a Waters World Centre of Innovation in HDXMS and a SCIEX Regional Centre of Distinction to other areas of mass spectrometry applications through partnerships with individual researchers and industrial collaborations.

Core Services

  • In-gel Protein Identification
    Identification of protein from gel bands/spots by MS
  • Shotgun Protein Identification
    Complex protein mixture (e.g. co-IP/pull-down sample)
  • Molecular Weight Determination
    Determining molecular weight of intact protein in solution by MS
  • Post-Translational Modifications
    Methods to study post-translational modifications such as sumoylation etc.

Collaborative Services

  • Phosphoproteomics
    Protein identification and phosphorylation analysis
  • Protein Structure, Dynamics and Interactions
    Amide hydrogen/deuterium exchange mass spectrometry (HDXMS)
  • Quantitative Proteomics
    Unbiased comparative quantification of proteomes using label-based (iTRAQ, iCAT, SILAC) or label-free (SWATH/DIA) technologies
  • Targeted Mass Spectrometry
    Selective relative/absolute quantification of target molecules using multiple reaction monitoring (MRM)

PPC PERSONNEL

STAFF

Recent Publications

Masson GR, Burke JE, Ahn NG, Anand GS, et al. (2019) Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments. Nat Methods. 16(7):595-602.

Hudgens JW, Gallagher ES, Karageorgos I, Anderson KW, Filliben JJ, Huang RY, Chen G, Bou-Assaf GM, Espada A, Chalmers MJ, Harguindey E, Zhang HM, Walters BT, Zhang J, Venable J, Steckler C, Park I, Brock A, Lu X, Pandey R, Chandramohan A, Anand GS, et al. (2019) Interlaboratory Comparison of Hydrogen-Deuterium Exchange Mass Spectrometry Measurements of the Fab Fragment of NISTmAb. Anal Chem. 91(11):7336-7345.

Ramesh R, Lim XX, Raghuvamsi PV, Wu C, Wong SM, Anand GS. (2019) Uncovering metastability and disassembly hotspots in whole viral particles. Prog Biophys Mol Biol. 143:5-12.

Sharma KK, Lim XX, Tantirimudalige SN, Gupta A, Marzinek JK, Holdbrook D, Lim XYE, Bond PJ, Anand GS, Wohland T. (2019) Infectivity of Dengue Virus Serotypes 1 and 2 Is Correlated with E-Protein Intrinsic Dynamics but Not to Envelope Conformations. Structure. 27(4):618-630.e4.

Ghosh M, Wang LC, Huber RG, Gao Y, Morgan LK, Tulsian NK, Bond PJ, Kenney LJ, Anand GS. (2019) Engineering an Osmosensor by Pivotal Histidine Positioning within Disordered Helices. Structure. 27(2):302-314.e4.

Purushothaman K, Das PP, Presslauer C, Lim TK, Johansen SD, Lin Q*, Babiak I*. (2019) Proteomics Analysis of Early Developmental Stages of Zebrafish Embryos. Int J Mol Sci. 20(24). pii: E6359.

Truong T, Zeng G, Lim TK, Cao T, Pang LM, Lee YM, Lin Q, Wang Y, Seneviratne CJ. (2019) Proteomics Analysis of Candida albicans dnm1 Haploid Mutant Unraveled the Association between Mitochondrial Fission and Antifungal Susceptibility. Proteomics. e1900240.

Das PP, Macharia MW, Lin Q*, Wong SM*. (2019) In planta proximity-dependent biotin identification (BioID) identifies a TMV replication co-chaperone NbSGT1 in the vicinity of 126 kDa replicase. J Proteomics. 204:103402.

Yao D, Liu Y, Chen X, Lim TK, Wang L, Aweya JJ, Zhang Y, Lin Q. (2019) In-depth proteomic profiling of the Singapore grouper iridovirus virion. Arch Virol. 164(7):1889-1895.

Gan HX, Zhou H, Lee HJ, Lin Q, Tong YW. (2019) Toward a Better Understanding of the Nature-Inspired Aquaporin Biomimetic Membrane. Langmuir. 35(22):7285-7293.

PPC map

Contact us

PROTEIN AND PROTEOMICS CENTRE (PPC)

S2 Level 2, Department of Biological Sciences
National University of Singapore
Singapore 117543

Campus map

  www.dbs.nus.edu.sg/ppc
  dbsbox32@nus.edu.sg
  +65 6516 4594 (through Wang Xianhui)
  +65 6516 7754 (through Lim Teck Kwang)