SINGAPORE LIPIDOMICS INCUBATOR (SLING)
Lipidomics@SingMass is the unit based at SLING in NUS and its goal is to advance the understanding of the biochemistry of lipids. We treat lipids not as isolated entities but as a metabolic network. By working with different research and corporate groups we will expand the knowledge further in this exciting and fast developing field.
SLING’s laboratory hosts a combination of mass spectrometers (low and high resolution), methods (shotgun and chromatography) and data analysis capabilities. This is supported by our knowledge of lipid pathway regulation in health and disease and in different organisms. We have developed several panels for robust, sensitive, quantitative and large-scale lipidomics.
Lipidomics@SingMass works as a collaborative service model. We help with project design, sample preparation, sample analysis and data interpretation.
- Shotgun untargeted
- LC targeted wide
- LC targeted
- Development of new panels
Over many years, SLING has developed and accumulated a unique set of experiences that apply state-of-the-art mass spectrometry for lipidomic analysis in various applications. These applications have been well documented in multiple peer reviewed journals.
Shared reference materials harmonize lipidomics across MS-based detection platforms and laboratories. Triebl A, Burla B, Selvalatchmanan J, Oh J, Tan SH, Chan MY, Mellett NA, Meikle PJ, Torta F, Wenk MR. J Lipid Res. 2019 Nov 15
Associations with metabolites in Chinese suggest new metabolic roles in Alzheimer’s and Parkinson’s diseases. Chai JF, Raichur S, Khor IW, Torta F, Chew WS, Herr DR, Ching JH, Kovalik JP, Khoo CM, Wenk MR, Tai ES, Sim X. Hum Mol Genet. 2019 Oct 19.
Large-scale lipidomics identifies associations between plasma sphingolipids and T2DM incidence. Chew WS, Torta F, Ji S, Choi H, Begum H, Sim X, Khoo CM, Khoo EYH, Ong WY, Van Dam RM, Wenk MR, Tai ES, Herr DR. JCI Insight. 2019 Jun 4;5
Changes in the Canine Plasma Lipidome after Short- and Long-Term Excess Glucocorticoid Exposure. Sieber-Ruckstuhl NS, Burla B, Spoerel S, Schmid F, Venzin C, Cazenave-Gassiot A, Bendt AK, Torta F, Wenk MR, Boretti FS. Sci Rep. 2019 Apr 12;9(1):6015.
MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelines. Burla B, Arita M, Arita M, Bendt AK, Cazenave-Gassiot A, Dennis EA, Ekroos K, Han X, Ikeda K, Liebisch G, Lin MK, Loh TP, Meikle PJ, Orešič M, Quehenberger O, Shevchenko A, Torta F, Wakelam MJO, Wheelock CE, Wenk MR. J Lipid Res. 2018 Oct;59(10):
Tear eicosanoids in healthy people and ocular surface disease. Ambaw YA, Chao C, Ji S, Raida M, Torta F, Wenk MR, Tong L. Sci Rep. 2018 Jul 26;8(1):11296
Mfsd2b is essential for the sphingosine-1-phosphate export in erythrocytes and platelets. Vu TM, Ishizu AN, Foo JC, Toh XR, Zhang F, Whee DM, Torta F, Cazenave-Gassiot A, Matsumura T, Kim S, Toh SES, Suda T, Silver DL, Wenk MR, Nguyen LN. Nature. 2017 Oct 26;550(7677):524-528.
Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum. Laursen T, Borch J, Knudsen C, Bavishi K, Torta F, Martens HJ, Silvestro D, Hatzakis NS, Wenk MR, Dafforn TR, Olsen CE, Motawia MS, Hamberger B, Møller BL, Bassard JE. Science. 2016 Nov 18;354(6314):890-893.
Mass spectrometry-based microbial metabolomics. Edward E.K. Baidoo, Peter I. Benke, Jay D. Keasling. Microbial Systems Biology, Methods in Molecular Biology, Humana Press (2012) 215-278